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Downregulation of REDD1 promotes A549 and H1299 cell apoptosis under hypoxia. ( A , B ) Apoptosis of A549 and H1299 cells following the knockdown of REDD1 was detected by flow cytometric analysis of Annexin V-FITC/PI. ( C , D ) Quantitation of apoptotic cells. ( E , F ) Protein expression of Bax and Bcl2 was determined by Western blot. n = 4. * p < 0.05; ** p < 0.01.

Journal: Biomedicines

Article Title: REDD1 Affects Proliferation, Apoptosis, Migration, and Colony Formation via p-ERK and p-JNK Signaling in Lung Adenocarcinoma Cells Under Hypoxia

doi: 10.3390/biomedicines13122918

Figure Lengend Snippet: Downregulation of REDD1 promotes A549 and H1299 cell apoptosis under hypoxia. ( A , B ) Apoptosis of A549 and H1299 cells following the knockdown of REDD1 was detected by flow cytometric analysis of Annexin V-FITC/PI. ( C , D ) Quantitation of apoptotic cells. ( E , F ) Protein expression of Bax and Bcl2 was determined by Western blot. n = 4. * p < 0.05; ** p < 0.01.

Article Snippet: B-cell lymphoma-2 (Bcl2) (A00040-1) antibody was obtained from Boster (Wuhan, China). p-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (#9101), p44/42 MAPK (ERK1/2) (#4695), p-SAPK/JNK (Thr183/Tyr185) (#4668), SAPK/JNK (#9252), p-p38 MAPK (Thr180/Tyr182) (#9211), p38 mitogen-activated protein kinase (MAPK) (#9212), p-Akt (Ser473), Akt (#9271), p-mTOR (Ser2448) (#5536), and mTOR antibodies (#2974) were acquired from Cell Signaling Technology Inc. (Beverly, MA, USA), while the β-actin (DKM9001L) antibody was obtained from Sungene Biotech Co., Ltd. (Tianjin, China).

Techniques: Knockdown, Quantitation Assay, Expressing, Western Blot